DusQ: New dark quencher series from Lumiprobe

Dark quenchers are non-fluorescent chromophores that can absorb excitation energy from a fluorophore and dissipate it as heat. Quenchers are widely used to synthesize oligonucleotides: hydrolyzable DNA probes, «molecular beacons», and «scorpion» primers.

Dark quenchers 此款淬滅劑是不具螢光的發色團，能吸收螢光基團的激發能量並將其以熱能形式散逸。廣泛應用於寡核苷酸的合成，例如：可水解 DNA 探針、molecular beacons 分子熒光探針、以及 scorpion 引子。

The use of quenchers in these applications has many advantages:
此款淬滅劑使用於上述之應用，具有許多優勢：

low background fluorescence and better signal-to-noise ratio;

背景螢光低，訊噪比更佳

high dynamic range;

動態範圍大

possibility of multiplexing since quenchers have no secondary fluorescence;

可實現多重檢測，因為淬滅劑本身沒有次級螢光

straightforward synthesis of DNA probes since quenchers are resistant to degradation at the deprotection stage.

DNA 探針合成更為簡單，因為淬滅劑在去保護（deprotection）步驟中具耐降解性

Below is a new series of DusQ® fluorescence quenchers with different spectral properties and functional groups for oligonucleotide synthesis and other applications.
以下為全新系列的 DusQ® 螢光淬滅劑，具備不同的光譜特性與官能基團，適用於寡核苷酸合成及其他應用。

DusQ®1

DusQ1 has an absorption maximum of 522 nm and an effective 480–580 nm absorption range. This dark quencher is preferred for use with fluorescent dyes in the yellow-green or yellow part of the spectrum (519–556 nm), such as FAM and TET.

DusQ®2

DusQ2 has an absorption maximum of 552 nm and an effective 560–670 nm absorption range. This dark quencher effectively quenches the fluorescence of dyes in the yellow-orange part of the spectrum (557–617 nm), such as HEX, JOE, TMR, TR, Cyanine3, and ROX.

DusQ®3

DusQ3 has an absorption maximum of 657 nm and an effective absorption range of 620–730 nm. This dark quencher is recommended for use with red and far-red fluorescent dyes (557–617 nm), such as BDP630/650, Cyanine5, Cyanine5.5, and AF 647.

DusQ®21

DusQ21 has an absorption maximum of 656 nm and an effective absorption range of 690–720 nm. This dark quencher can be used as an alternative to DusQ3 for coupling with red and far-red fluorescent dyes (557–617 nm), such as BDP630/650, Cyanine5, Cyanine5.5, and AF 647.

Comparison of spectral properties of DusQ® dark quenchers:
DusQ

More fluorescence quenchers with different functional groups →

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